Leica SP8 3X STED Confocal/Super-Resolution Microscope
$30/hour ($90/hr for industry users)
Room: 2074 WIMR2
The SP8 is both a point scanning confocal and 3X STED super-resolution microscope. The SP8 includes a super-continuum white-light laser for fluorescent excitation from 470 to 670 nm, a separate 405 nm diode laser, and 3 depletion lasers (592 nm/660 nm/775 nm) for performing 3 color super-resolution STED imaging. The unit is equipped with 3 PMTs and 2 highly sensitive HyD detectors for image collection. Additional features include a motorized stage, resonant scanner, fluorescence spectral detection and a super Z piezo stage/Tokei Hit insert for live cell imaging. Objective lens: 10x, 20x, 40x, 63x, 63xWi and 100x.
| wdt_ID | Magnification | Type | Immersion | NA | Working Distance |
|---|---|---|---|---|---|
| 1 | 10x | PLAN APO | Dry | 0.4 | 2.2mm |
| 2 | 20x | PLAN APO | Dry | 0.75 | 0.62mm |
| 3 | 40x | PLAN APO | Oil | 1.3 | 0.24mm |
| 6 | 63x | PLAN APO | Oil | 1.4 | 0.14mm |
| 7 | 100x | PLAN APO | Oil | 1.4 | 0.1mm |
Nikon A1RS HD Confocal Microscope
$30/hour ($90/hr for industry users)
Room: 2075 WIMR2
The A1 is an inverted point scanning confocal microscopy system with six excitation laser lines: 405 nm, 440nm, 488 nm, 514nm, 561 nm, and 638 nm. Additional features include high sensitivity GaAsP detectors, a resonant scanner, fluorescence spectral detection and a Tokei Hit insert for live cell imaging. Objective lens: 4x, 10x, 20x, 40x, 60x, 60x (water immersion), and 100x.
| wdt_ID | Magnification | Type | Immersion | NA | Working Distance |
|---|---|---|---|---|---|
| 1 | 10x | PLAN FLUOR DL | Dry | 0.3 | 15.2mm |
| 2 | 20x | PLAN APO VC | Dry | 0.75 | 1mm |
| 3 | 60x | PLAN APO VC | Water | 1.2 | 0.31-0.28mm |
| 4 | 60x | PLAN APO VC | Oil | 1.4 | 0.13mm |
| 5 | 100x | PLAN APO | Oil | 1.4 | 0.13mm |
| 7 | 4x | PLAN APO | Dry | 0.20 | 20 mm |
Nikon A1R HD Upright Multi-Photon/Confocal microscope
$30/hour ($90/hr for industry users)
Room: 2076 WIMR2
Nikon Upright FN1 microscope equipped with Dual Multi-Photon lasers (Coherent Discovery) tunable to 1300nm and 4 excitation laser lines (405nm, 488nm, 561nm, and 638nm) for confocal imaging. Stage is equipped with Intravital Pad, Piezo insert and Tokei Hit biochamber. For both visible confocal and multiphoton imaging, photon collection utilizes high sensitivity GaAsP detectors.
Please credit grant number 1S10OD025040-01 in resulting publications through your NCBI account. These publications should be visible in NIH RePORTER and linked with the S10 award if done correctly.
| wdt_ID | Magnification | Type | Immersion | NA | Working Distance |
|---|---|---|---|---|---|
| 2 | 4x | PLAN APO | Dry | 0.20 | 20 mm |
| 3 | 10x | PLAN APO | Dry | 0.45 | 4.0 mm |
| 4 | 16x | LWD | Water | 0.80 | 3.0 mm |
| 6 | 25x | APO LWD | Water | 1.10 | 0.17 mm |
| 7 | 40x | NIR APO | Water | 0.8 | 3.5 mm |
Nikon Ti2 Spinning Disk Confocal Microscope
$30/hour ($90/hr for industry users)
Room: 2072 WIMR2
Blue/Green/Red/Far Red filter sets for 4 color spinning disk confocal microscopy. The Yokogawa CSU-W1 is the standard tool for live cell imaging due to its fast scanning and low photo-bleaching capability. The W1 provides a wider field of view as compared to previous spinning disk confocal microscopes and uses 2 Hamamatsu Orca Flash 4 cameras. The system includes a motorized stage, generation 4 Perfect Focus System and Tokei Hit for live cell imaging. Objective lens: 10x, 20x, 40x, 60X and 100x.
| wdt_ID | Magnification | Type | Immersion | NA | Working Distance |
|---|---|---|---|---|---|
| 1 | 10x | FL PLAN | Dry | 0.25 | 17.6 mm |
| 2 | 20x | PL FLUOTAR | Dry | 0.5 | 1.15 mm |
| 3 | 40x | PL FLUOTAR | Dry | 0.75 | 0.4 mm |
| 4 | 63x | PLAN APO | Oil | 1.4 | 0.14 mm |
| 5 | 100x | PLAN APO | Oil | 1.4 | 0.09 mm |
Mizar Tilt
$30/hour ($90/hr for industry users)
Room: 2073 WIMR2
Mizar Imaging’s Tilt is a modular light sheet illumination system enabling high-speed, high-resolution, and 3D light sheet imaging on the Leica DMi8 inverted microscope. Equipped with 488, 561 and 647 emission lasers, it is ideal for high-resolution imaging while reducing photobleaching and phototoxicity. The setup features a high-resolution Prime 95B camera, and a Leica color camera and the following objective lenses: 5X, 10X, 20x, 40x, 60x, and 100x. This system also includes an environmental chamber for live-cell imaging.
3i Lattice Lightsheet
$30/hour ($90/hr for industry users)
Room: 2071 WIMR2
The 3i Lattice Lightsheet utilizes Bessel beam lattice light sheet illumination. This enables high spatiotemporal resolution 4D live cell imaging while reducing phototoxicity and photobleaching. The setup is equipped with a Hamamatsu ORCA-Flash4.0 V3 sCMOS high-resolution camera, and features a controlled temperature and CO2 incubator for live-cell imaging. This unit will be equipped with 488, 560 and 642 excitation lasers and a 25x/1.1NA water immersion objective.
