Microscopes

Leica SP8 3X STED Confocal/Super-Resolution Microscope

$30/hour ($90/hr for industry users)

Room: 2074 WIMR2

The SP8 is both a point scanning confocal and 3X STED super-resolution microscope. The SP8 includes a super-continuum white-light laser for fluorescent excitation from 470 to 670 nm, a separate 405 nm diode laser, and 3 depletion lasers (592 nm/660 nm/775 nm) for performing 3 color super-resolution STED imaging. The unit is equipped with 3 PMTs and 2 highly sensitive HyD detectors for image collection. Additional features include a motorized stage, resonant scanner, fluorescence spectral detection and a super Z piezo stage/Tokei Hit insert for live cell imaging. Objective lens: 10x, 20x, 40x, 63x, 63xWi and 100x.

wdt_ID	Magnification	Type	Immersion	NA	Working Distance
1	10x	PLAN APO	Dry	0.4	2.2mm
2	20x	PLAN APO	Dry	0.75	0.62mm
3	40x	PLAN APO	Oil	1.3	0.24mm
6	63x	PLAN APO	Oil	1.4	0.14mm
7	100x	PLAN APO	Oil	1.4	0.1mm
8	63X	PLAN APO	water	1.2	.3 mm

Nikon AXR Confocal Microscope

$30/hour ($90/hr for industry users)

Room: 2075 WIMR2

The UWOIC asks that publications benefitting from the use of the Nikon AX credit the grant number 1S10O34394-01.

Please reference the AXR SIG NIH award number “S10OD034394-01” into your NCBI/PubMed account.

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Manufacturer Info

The AXR is an inverted point scanning confocal microscopy system with eight excitation laser lines: 405 nm, 440nm, 488 nm, 514nm, 561 nm, 594nm, 640nm and 735nm. Additional features include high sensitivity GaAsP detectors, a resonant scanner, and a Tokei Hit enclosure for live cell imaging. Objective lens:2x, 10x, 20x, 40x dry, 40x oil, and 60x oil.

wdt_ID	Magnification	Type	Immersion	NA	Working Distance
1	2x	PLAN APO Lambda D	Dry	0.1	8.5 mm
2	20x	PLAN APO Lambda D	Dry	0.8	0.8 mm
3	10x	PLAN APO Lambda D	Dry	.45	4 mm
4	40x	PLAN APO Lambda D	dry	0.95	.25
7	40x	PLAN Fluor Lambda D	Oil	1.3	.2 mm
8	60x	PLAN APO Lambda D	Oil	1.42	.13
9

Nikon A1R Confocal Microscope

$30/hour ($90/hr for industry users)

Room: 2073 WIMR2

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Manufacturer Info

Nikon A1R inverted Ti2 microscope equipped with 4 excitation laser lines (405nm, 488nm, 561nm, and 638nm) for confocal imaging. ** Confocal fluorescence only (no transmitted light capabilities)

wdt_ID	Magnification	Type	Immersion	NA	Working Distance
2	4x	PLAN APO	Dry	0.20	20 mm
3	10x	PLAN APO	Dry	0.45	4.0 mm
4	20x	Plan Apo	dry	0.80	1 mm
6	40x	Plan Apo	Oil	1.3	.250 mm
7	60x	Plan Apo	Oil	1.4	.130 mm
8	60x	Plan Apo	water	1.2	.3 mm

Nikon Yokogawa W1 CSU Spinning Disk Confocal Microscope

$30/hour ($90/hr for industry users)

Room: 2072 WIMR2

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Manufacturer Info

Blue/Green/Red/Far Red filter sets for 4 color spinning disk confocal microscopy. The Yokogawa CSU-W1 is the standard tool for live cell imaging due to its fast scanning and low photo-bleaching capability. The W1 provides a wider field of view as compared to previous spinning disk confocal microscopes and uses 2 Hamamatsu Quest cameras. The system includes a motorized stage, generation 4 Perfect Focus System and Tokei Hit enclosure for live cell imaging. Objective lens: 10x, 20x, 40x, 60X and 100x.

wdt_ID	Magnification	Type	Immersion	NA	Working Distance
1	4x	Plan Apo	Dry	.2	20
2	10x	Plan Apo	Dry	.45	10mm
3	20x	Plan Apo	Dry	0.8	.8
4	40x	Plan Apo	dry	.95	0.25
5	60x	Plan Apo	Oil	1.4	.130 mm
6	100x	Plan Apo	Oil	1.4	0.09 mm

Bruker Ultima Multi-Photon Microscope

Room 2076WIMR2

Inverted and Upright Platforms

16x, 25x, 40x Wi objectives

Coherent Discovery 2 Photon laser with 680-1300nm range

3i Lattice Light Sheet

Room 9001WIMR2

Slidebook software

Laser sources 488, 561 and 640nm